Coding

Part:BBa_K2331001:Design

Designed by: Jacob Sicheri   Group: iGEM17_Dalhousie   (2017-10-17)


Endoxylanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2578
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1999


Design Notes

Certain codons of the part had to be modified to comply with the biobrick cloning requirements.



Source

This part was identified from a synthetically derived metagenomic library of the porcupine microbiome. After identification, the gene was synthesized by IDT DNA. It has closest similarity to Bacteriodes spp.


References